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Axopacht 200b is the latest version of the top axon industries membrane amplifier, with the use of innovative capacitors feedback techniques for single-channel recording, resistance feedback techniques for whole-cell recording, and optimal performance-cell membranes for single-channel and whole-cell recording. Convenient functions include zap (disruption of stickers throughout the battery), double-speed current clamps (permissible for faster biting of currents in small batteries), maintenance orders (used for voltage commands in voltage clamps and current commands in current clamps), and the selection of three add-on settings on dedicated current output (used for stickers, cell-wide and loose sheet modes). Axopattch 200b provides a single channel recording of the lowest noise. 0. 13 pa rms (10 khz) alarmingly low-open noise increases to 0. (b) 145 pa rms when the frame of the sticker is connected to the entry end of the probe and the capacitor's face is fully compensated (with the elimination of the electric charge transient). The power of the electric feedback technology is as follows (figure 1): room-temperature feedback is clearly better than resistance feedback technology; cooling it is better! Unprecedented achievements in the field of ultra-low noise recording
For manual use
The double probe of the double-layer experiment shall remain stable under the large input capacity load. Axopattch 200b is solid as a rock and enters an electric cap of 1000 pf. In addition, the integration technology in axopattch 200b allows double-layer voltage to move very quickly. There are two reasons. First, it has an intrinsically large dynamic range, allowing it to pass through a few nuanced currents. Second, during a large command pressure step, the separator enters the state of consolidation. In this state, the probe continues to work as a voltage clamp amplifier, but the feedback resistance is very low. Within a few milliseconds of the compound, it can be recharged rapidly to the double layer by currents up to 1 ma。
== sync, corrected by elderman == @elder manExcellent whole-cell performance
Retarded probe mode
In cytological records, cells and the environment produce more current noise than amplifiers. As a result, the advantages of low-noise feedback probes cannot be effectively utilized in a cell-wide pattern. For this reason, the axopatch 200b uses the traditional electrical device of resistance feedback for film plier recording. Cv 203bu probes include two feedback resisters that provide extensive current passivity in battery-wide mode. 500 meu feedback resistance (ß1) provides low noise and large current passivity (20 na). For larger currents, you can switch to 50 meu feedback resisters (ß0. 1) to transmit currents up to 200 na。
Serial resist compensation
There are two objectives, projections and corrections。
Serial resistance prediction
The membrane level reaches its specified value as quickly as possible by increasing the size of the command pressure step leap ("pressure"。
Serial resist correction
In order to eliminate errors caused by voltage reductions on electrodes and to increase the bandwidth of records, positive feedback is applied to increase command level by adding signals proportional to the measurement of currents to the command input. Serial resistance prediction and correction can be used together or separately on axopattch 200b。
Double-speed current clamp
Axopattch 200b uses double-speed current clamps to optimize speed and stability. The choice of speed depends on the resistance of the fluid transferor. I-clamp fast can be used to obtain 10 ms of uptime under many experimental conditions for transporter resisters exceeding 10 m. The i-clamp normal model ensures the stability of any transporter resistance exceeding 1 mega-eu. In addition, current to zero current (i=0 mode) and slower to zero current (track mode) can be used to track the slow movement of the fluid transporter when close to the cell before sealing up. In addition, axopattch 200b, which includes chain resistance compensation in current clamps, allows correction of voltage errors due to the electrical resistance of the mover。
== sync, corrected by elderman == @elder manEmbracing feedback film clamps
Axopattch 200b provides minimal noise membrane amplifiers. The open-way (magnifying) noise under the sticker mode has been reduced to an unprecedentedly low level: < 15 femtoamps (rms), < 60 femtoamps (rms) and < 130 femtoamps (rms) khz bandwidth below 5 khz bandwidth below 10, all measured by 8 peptéceers. The noise remains low with the installation of the liquid transferer stubs (10 khz bandwidth below 145 femtoamps rms). This means that the noise is lower during the actual recording; this noise performance is partly achieved by cooling the input field effect transistor within the probe to a level far below 0°c。
Better noise performance is just part of the story. The redesigned super thin probe improved electrodes ' access to preparation by making it easier to install it under a microscope. We now have two full cell ranges in one probe (previously available only in two separate probes)。
== sync, corrected by elderman == @elder manIncluding
Axopach 200b with the following items:
- Axoptch 200b amplifier
- Cv-203bu detector (part 1-cv 203bu)
- U. S. Power line (parts 1-3010-0011)
- European power line, cee7/7 (parts 1-3010-0013)
- Annex packages (components 1-2050-0301) consist of: acc fuses (components 1-2050-0014), panels (components 1-5200-0100), electrode supports (components 1-hl-u), series of electrical resistance vibrating boxes (components 1-dr-1), patch-1u units (components 1-patch-1u), manuals (components 1-2500-0121)
Mover offset
Axopattch 200b provides a offset of 250 mv。
Seal test
Convenient sealing tests can be used for voltage clamp mode (5 mv pulse) or current clamp mode (50 pa (ß1) or 500 pa (ß0. 1)。
Double command level
The two stand-alone command level input allows you to add a signal from two different sources. The back plate commands are scaled to provide a wider range (up to ±1v) and are therefore very useful for electrochemical measurements。
Maintain command
The axopattch 200b maintenance command is enhanced by adding an x1 and x5 switch, allowing you to select a range of 0 – 200 mv or 0-1 v. When an external command from a computer is used to establish a maintenance level, the on/offf switch can disable this control。
Capability compensation for the fluid transferer
Controls the range and tau of the two-time constant fast and slow when operating in voltage or current plier mode。
Cell capital corporation
Compensation is up to 100 pf (ß1) or 1,000 pf (ß=0. 1) to allow recording from a wide range of unit sizes。
Output gain
You can select ten-gain settings across a 1,000-fold range to scale the output to the optimal level。
Leak reduction
Axopattch 200b allows you to correct leakage errors without adjusting the data acquisition software settings。
It's low
The filter 4 polar bézier filter with five settings from 1 khz to 100 khz allows you to set up access suitable for your application within twice the previously available range。
Panel meters
Report current, voltage, or spot temperature values under any operating mode. A steady current is reported to monitor voltage plier output. To assess noise, currents are shown as rms values。
Zap
Axopattch 200b promotes the breakup of the stickers by applying a single +1. 3v pulse for the selected duration。
Squirer support
Provides two hl-u stafts of different lengths that can accommodate liquid transporters with an outer diameter of 1. 0 mm to 1. 7 mm。