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Selected high-quality documents - expensive for you rt-pcr principles and experimental step one, knowledge background one, genetic expression: dna rnaprotein's single-copy gene expression has a gradual magnification mechanism, such as a silk-coring protein gene, 104 silk protein mrnas (4d per mrna survives with 105 silk proteins). So the mrna expression level of a single copy gene is important for the regulation of its functional level. Pcr technology (polymerasechainreaction): is the polymeric chain reaction. Subject to the presence of templates, quotations and four deoxynucleic acids, reliance is placed on the enzymes reaction of dna polymerase, the specificity of which is determined by the sequence of two synthetic quotations. Reactions in three steps: a. Variables: distinction of the hydrogen key of the double-heated dna to form a single chain of dna by heating; b. Repulse of fire: cooling the reaction mixture to a certain temperature, combining the quotation with the template. C. Extension: with the presence of dna polymerases and dntps and mg2, the deflammatories extend in the direction of 5 3. The above three steps are a cycle, so repeated. 3. ReversetransCriptas
