Paraffin cut-off protocol 1. Purpose
Regulate technical handling of paraffin slices。
2. Operational procedures
The tissue of waxes made of paraffin wrappings is known as the pheasant slice method using a slice mechanism. Slicing methods are commonly used for current pathological diagnosis. 2. 1 preparation of slices
2. 1. 1 post-fixed specimens are made of wax blocks after dehydration, transparency, immersion and burial. High-quality wax and sharp blades are key components in ensuring the quality of the slices。
2. 1. 2 preparation of adequate treatment of clean-carrying tablets and thermocalcissors, high- and medium-quality hyena pens and pencils (for rough end numbers on the film)
2. 2 slice production process
2. 2. 1 pre-corrected wax blocks are cooled in a ready ice box and subsequently fixed on the head of the slicer. Slice blades placed on a knife rack with a blade and wax on a surface of 5o. The wax is adjusted to the right position with the knife, and the blade is moved to the frame or wax fixator so that the wax is in contact with the blade。
2. 2. 2 the slices use more wheeled slicers, use more hand-held brushes, and the small specimens are not repaired too much to prevent the satisfactory slices from being cut, and the large ones should have been completed. After cutting out the wax, lift it lightly with a pencil and then pick it up with an eyebrush, and put the projector up front (with the display temperature adjusted to the melting point of the paraffin used, usually 35-50°c), before the slice can be divided and recovered. Slices can be used for 30 per cent of the initial alcohol exhibition, and then used to pick up the film and put it on the exhibit. In order to reduce the amount of heat generated by sliced blades and tissue blocks in the course of the slices and to keep paraffins to a suitable hardness, the slices can often be used to cool tissue blocks with ice, especially during the summer hot season。
2. 2. 3 marking of slices: a pencil with the number of the specimen and the number of the wax block on the slice, indicating the subject number, if necessary。
3. Attention
3. 1 a rotor slicer should cut the tissue, so that, in order to prevent a cleavage in the tissue, the hard-to-cut part of the tissue should be upper (e. G., skin tissue, the skin section should be up). In the case of tissues such as gastrointestinal, the membrane should be up。
3. 2 be careful where you are, leave room for tagging and look good. When you pick up the slice, write the number。
When the slices are recovered, they can be roasted with a slight drying in the air, usually at 48°c and 30°c or stored in a 37°c oven. However, for larger organizations, it is necessary to dry them completely before they can bake. Otherwise, bubble effects may be dyed。
Schedules
Schedule 1 pathological specimens, typhus sheet。
