Bee poisoning is a transparent venom of aromatic aroma from the worker bee and adjoining glands, stored in a venom and excreted from a needle when stinging. Its chemical composition is complex and includes polypellium, enzymes and non-pellium substances. The main active ingredients are bee toxin, bee-toxic nephramine, phosphosterase a2, transparent acidase, dopamine and tissueamine. Of these, bee toxin is the main ingredient of bee poison, which is about 50 per cent of its dry weight. The bee toxin is a small molecule consisting of 26 amino acids, with a relative molecular mass of 2849, a strong alkaline combined molecule, which is soluble in water. The bee toxin is highly biologically active, and modern pharmacological research shows that it has multiple effects, such as resistance to inflammation, pain, inhibition of blood plate condensation, anti-aids and anti-tumours. In recent years, the anti-tumour effects of bee poisons and their potential value for clinical applications have received increasing attention。
Because bee toxin toxicity is more pronounced, high doses are used with some toxic effects to normal tissues, especially soluble reactions, limiting its extensive application in oncology. For the purpose of detoxification efficiency, studies have been carried out to produce bee toxin using polosham 188LipidAs a membrane surface decorator, its polyoxyethylene prosthesis is embedded in the phosphorus double-molecule layer, which reduces the decomposition of plasma protein, reduces oscillation and prolongs the length of the drug's stay in blood for the purpose of detoxification. Compared to the anti-tumour effects of the different formulations of bee toxin, it was found that bee toxin lipids were significantly less toxic than pure bee toxin, that tumour growth in bee toxin lipid treatment groups was significantly inhibited, that tumour tissues were widespread, that the average life of the treatment was prolonged, and that the combination effects were better than that of simple bee toxin groups. It is shown that bee toxin lipids increase their time of effect on tumour cells, increase their anti-tumour effects, and reduce the toxicity of bee toxin to the organism, demonstrating a clear detoxification synergy。
Mechanisms for inhibiting the growth of tumours by bee toxin: experimental studies on oncological and therapeutic effects of hive toxin on the rat tumour model (s180) have found direct and some therapeutic effects on the tumour cells of mice. A bee toxin, the main active ingredient of bee poisoning, is harmful to a variety of experimental tumours, and a bee toxin at 1 mol/l concentrations prevents the growth of tumor cells without inhibiting normal cell growth and cloning rates. It is generally assumed that bee toxin dissolves the cytochrome, inhibits the normal breathing of the cell, inhibits the process of tumour tissue oxidation of phosphoric acid and disrupts tumour tissue growth. 2. Immunization regulation. A long-standing foreign study has reported the involvement of bee toxin in anti-tumour t cell responses. Hive 0. 32, 0. 63 mg/kg increases the lymphocyte esterase positive chromosomal rate of s180 rats, indicating an enhanced function of t lymphocytes. Hive 0. 32 mg/kg enhances the mesh-like inner cortex system (res) of s180 botulinums for carbon profiles and enhances the oscillation function of the res mononucleus cells. It is therefore assumed that bee poison is used for anti-oncological treatment and that its mechanisms may be associated with enhancing the osmosis function of the res and enhancing the immune function of cells. Since bee poisoning is a positive adjustment to the immune function of the organism by enhancing the functioning of the cell of the cell of the cell of the cell of the cell of the organism, it is possible to enhance the immune function of the cell of the organism of the cell of the cell of the cell of the cell of the cell of the organism of the cell of the cell of the cell of the cell of the organism of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the cell of the type of the cell of the cell of the cell of the cell of the type of the cell of the cell of the organism, it is therefore important to conduct studies to treat diseases such as low immunopathy of the cell of the cell of the cell of the cell of the cell of the cell of the disease of the cell of the cell of the disease of the treatment of the disease of the disease of the disease of the tum of the cells of the cells of the disease of the tum of the tumour, of the tumour and the infection of the virus of the virus of the virus of the since then, studies of the effects of bee poisoning on t-lymphocytes have shown that bee poisoning is a two-way regulation of t-lymphocytes, showing a tendency to return to normal range regardless of direction, while it has little impact on patients with t-lymphocytes in normal range, considering bee poisoning to be an ideal immunomodifier. 3. Inducing tumour cells to die. The occurrence and development of tumours are not only the result of cell proliferation and fragmentation anomalies, but are also related to the disruption of cell decay. Cell decay is a different way of death from necrosis of cells. It is a biological death of cells controlled by genes, which, like cell proliferation and fragmentation, is a genetically controlled active cell biology process. New advances have been made in recent years in research on mechanisms to combat tumours of bee poisoning and its main ingredient, and numerous studies have found that the bioactivity of bee poisoning and bee toxin against tumours has been achieved by inducing tumour cells to die. Beetoxin inhibits the growth and induces the death of human liver cancers bel-7402, breast cancer cell lines mcf-7, t lymphocyte leukemia 6t-cem, etc。
Purpose of the study
This experiment will study the internal and external tumour resistance of bee toxin lipids and determine their efficacy in cell and mouse tumour models. This study begins with a clinical pre-drug ergonomic study of bee toxin lipids, both in and out of the body, to determine their potential therapeutic effects and advantages of detoxification efficiency vis-à-vis the bee toxin monomer; and a more in-depth exploration of its immune regulation mechanisms for treating tumours, which gives us a better understanding of the process of their functioning and of targets in the immune response network, and leads to better clinical research。
Iii. Proposed research programme
3. 1 mtt detects amphibious inhibition of bee toxin lipids on the tumour strain of bel-7402 and ic50。
(1) cell digestion, counting, vaccination in 96 holes, cell density: 0. 8 to 1. 5104/100m;l
The rest is hidden, you need to pay $10 to see the article in its entirety
